| Types | DnaRegion
|
| Roles | engineered_region
Signalling
|
| Sequences | BBa_K1180002_sequence (Version 1)
|
Description
This part is designed to direct exosome to the brain. Lamp 2b (lysosomal associated membrane protein 2b) is a protein ubiquitously expressed on the surface of the exosomes. By genetically engineer a target protein to the outmembrane part of the lamp 2b, we can use the lamp 2b to bring our target protein to the surface of the exosome. Thus we can endow the exosome with site-specificity by the addition of the fusion protein.For brain targeting, we choose to use RVG, which is a short peptide from Rabies Virus, as our target protein. RVG can specifically recognize acetylcholine receptor in the central nervous system, thus we engineer the RVG peptide into the lamp 2b and we use pcDNA 3.1(+) as our vector.
Notes
We need to make the RVG peptide as short as possible to avoid the immune response which might be induced by the fusion protein.
Source
The sequence is from the protein lamp 2b and RVG peptide.
