Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Eric Pederson
Date created: 2013-09-03 11:00:00
Date modified: 2016-01-25 02:40:37
KillerRed
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1184000_sequence (Version 1) |
Description
KillerRed is a red fluorescent protein that produces reactive oxygen species (ROS) in the presence of yellow-orange light (540-585 nm). KillerRed is engineered from Aequorea victoria GFP (avGFP) to be phototoxic. It has been shown that KillerRed produces superoxide radical anions by reacting with water. Superoxide reacts with the chromophore of KillerRed, causing it to become dark, which ultimately gives rise to a bleaching effect. KillerRed is spectrally similar to mRFP1 with a similar brightness. KillerRed is oligomeric and may form large aggregates.This sequence is codon optimized for mammalian cells and has 14 rare proline codons for E. coli (CCC) and one rare arginine codon (AGA). Codon optimization should be taken into consideration if large amounts of the protein are required. Expression on a high-copy plasmid has produced detectable fluorescent signal, however.
Notes
This sequence is not codon optimized for E. coli.Source
Engineered from avGFP. Provided by the Bruchez lab at Carnegie Mellon| Sequence Annotation | Location | Component / Role(s) |
| Double Stop Start cds Phototoxic Mutation Glu(70) Phototoxic Mutation Ser(121) Chromophore Phototoxic Mutation Asn(147) Phototoxic Mutation Ala(163) | 718,723 1,3 1,717 208,210 367,369 199,207 439,441 487,489 | feature/stop stop_codon start_codon feature/start feature/cds CDS sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |