EHEC_TALE1

BBa_K1189032 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1189032
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ali Honarmand
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:09:38

EHEC TALE1 (binds to 19bp of stx2 gene)



Types
DnaRegion

Roles
engineered_region

Generator

Sequences BBa_K1189032_sequence (Version 1)

Description

This TALE is designed by iGEM Calgary's 2012 team. It is designed in a way to bind to a 19bp region of the Stx2 gene found in EHEC organisms.

Notes

A KasI restriction cut site is incorporated at the end of the part so that we can swap the TALEs we currently have in our parts with this one.

Source

this part is being synthesized using IDT G-blocks.

Sequence Annotation Location Component / Role(s)
R0010
RFC10_codingScar
hisTag
B0034
EHEC_TALE1
KasI
Kcoil
GS_linker
1,200
221,226
227,248
209,220
249,2662
2546,2551
2597,2659
2552,2596
feature/promoter promoter
sequence_feature feature/misc
feature/cds CDS
feature/rbs ribosome_entry_site
CDS feature/cds
feature/misc sequence_feature
feature/misc sequence_feature
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1189032/1