BBa_K1189033

BBa_K1189033 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1189033
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ali Honarmand
Date created: 2013-09-26 11:00:00
Date modified: 2015-05-08 01:09:38

EHEC TALE2 (binds to a 19bp region of stx2 gene)



Types
DnaRegion

Roles
Generator

engineered_region

Sequences BBa_K1189033_sequence (Version 1)

Description

This TALE is designed in a way to bind to a 19bp region of the Stx2 gene found in EHEC organisms.

Notes

A KasI restriction cut site has been incorporated in the end of the TALE, so that part can easily be used to replace the TALEs in our other constructs.

Source

This part is being constructed using IDT G-Blocks.

Sequence Annotation Location Component / Role(s)
BBa_J04500
BBa_R0010
BBa_B0034
hisTag
EHEC TALE2
GS_linker
K_coil
KasI cut site
1,220
1,200
209,220
227,247
248,2551
2552,2596
2597,2659
2546,2551
feature/BioBrick engineered_region
feature/promoter promoter
ribosome_entry_site feature/rbs
feature/cds CDS
CDS feature/protein
CDS feature/protein
feature/cds CDS
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1189033/1