Up RcnA

BBa_K119007 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K119007

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Libertad Pantoja

Date created: 2008-09-28 11:00:00

Date modified: 2015-05-08 01:09:38

Upper primer for RcnA

Details

• Types: DnaRegion
• Roles: primer; Primer
• Sequences: BBa_K119007_sequence (Version 1)

Description

Upper primer used to add cI promoter upstream of the RcnR operator in the device cI+AVGreg_RcnRop_RcnA()

Notes

A prediction of the RcnR operator site was used to construct this part, we needed a repressor to control it, so we added the cI promoter upstream of the RcnR operator.

Source

Genomic DNA from E.coli and cI promoter.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051	1,49	engineered_region, feature/BioBrick
OR2	1,17	feature/binding, non_covalent_binding_site
-35	15,20	promoter, feature/promoter
OR1	25,41	non_covalent_binding_site, feature/binding
-10	38,43	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K119007/1

Member of these Collections

• iGEM Parts Registry