Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Osbaldo Resendis, Miguel A. Ramirez, Mariana G. Schiavon, Libertad Pantoja,Martin del castillo Velasco,Mariana B. Reyes, Luis A. Arriola, Daniela Robles, Sur Herrera, Carlos Vargas, Jimena Monzon S, E
Date created: 2008-09-30 11:00:00
Date modified: 2015-05-08 01:09:38
LuxR_cI_aiiA_Normal lacZ promoter
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K119010_sequence (Version 1) |
Description
This device encodes cI, the protein which controls RcnA. It also contains LuxR, which Induces the expression of cI and aiiANotes
We needed a repressor which could be induced by a "signal" in order to inhibit the RcnA(BBa_K119003, BBa_K119009) activity. We achieved this by taking advantage of the LuxR promoter present in the Chiba device BBa_I729006. So, we placed cI downstream of this promoter and changed the promoter which was controlling aiiA for the LacZ one.Source
We based this part in BBa_I729006 from Chiba team| Sequence Annotation | Location | Component / Role(s) |
| TetR 1 BBa_R0040 -35 TetR 2 -10 BBa_B0034 conserved BBa_C0062 prefix luxR BBa_B0010 stem_loop BBa_B0012 T7 TE polya stop BBa_R0062 LuxR/HSL -35 -10 RBS-3\Weak BBa_B0032 RBS BBa_C0051 cI lambda LVA LacZ promoter BBa_K119000 BBa_B0034 BBa_C0060 start aiiA LVA 2 BBa_B0010 stem_loop BBa_B0012 polya stop | 1,19 3,22 21,27 28,56 45,50 65,76 67,70 83,863 83,84 83,833 872,951 883,926 960,1000 967,986 987,1000 993,995 1009,1063 1009,1029 1028,1033 1050,1055 1072,1084 1072,1084 1078,1081 1089,1838 1089,1799 1800,1838 1845,1882 1845,1882 1889,1900 1907,2720 1907,1909 1907,2656 2686,2718 2690,2692 2729,2808 2740,2783 2823,2843 2844,2857 2850,2852 | non_covalent_binding_site feature/binding feature/BioBrick engineered_region feature/promoter promoter feature/binding non_covalent_binding_site promoter feature/promoter feature/BioBrick engineered_region feature/conserved conserved_region engineered_region feature/BioBrick engineered_region feature/BioBrick CDS feature/cds engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site stop_codon feature/stop engineered_region feature/BioBrick feature/binding non_covalent_binding_site feature/promoter promoter promoter feature/promoter feature/rbs ribosome_entry_site feature/BioBrick engineered_region ribosome_entry_site feature/rbs engineered_region feature/BioBrick feature/cds CDS tag feature/tag promoter feature/promoter engineered_region feature/BioBrick engineered_region feature/BioBrick engineered_region feature/BioBrick start_codon feature/start feature/cds CDS feature/tag tag feature/stop stop_codon engineered_region feature/BioBrick feature/stem_loop stem_loop feature/BioBrick engineered_region feature/polya polyA_site stop_codon feature/stop |