Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rohan Bendre, Aman Kumar, Namit Holay, Mitan Sutradhar, Kanishka Waghmare, Nishita Mohan, Nandita Damaraju, Mayank Choudhary
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:38
pLuxR --> ompF --> Gb3 mimic
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1194000_sequence (Version 1) |
Description
Gb3 mimic codes for a nine-amino acid peptide that has anti-Shiga toxin activity. It is coded downstream of ompF which is an periplasmic translocation signal peptide. The entire assembly is under control of an N-acyl homoserine lactone (AHL) dependent pLuxR promoter.The part, when translated, produces the Gb3 mimic peptide. The ompF signal peptide helps to transport Gb3 mimic into the extracellular space. Gb3 mimic neutralises the Shiga toxin by binding to it and sequestering it.
Other teams can utilise the ompF signal peptide if they want to ensure extracellular secretion of their protein of interest, especially if it is a small peptide. The Gb3 mimic peptide, though specific for the Shiga toxin, can be used for toxicology studies in general. Particularly, it can be of use to create a peptide library to investigate anti-toxin activities of therapeutic peptides by molecular docking.
Notes
We had the amino acid sequence of the Gb3 mimic. Since we wanted to express our peptide in Escherichia coli (N99 strain), we performed codon-optimisation on the Gb3 mimic DNA before designing the construct.Source
ompF signal peptide is derived from Escherichia coli.Gb3 mimic peptide is a synthetic peptide which comes from a peptide library.
pLuxR is a previous Registry part.
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR 1 -35 TetR 2 -10 BBa_B0034 conserved prefix BBa_C0062 luxR T A Help:Barcodes BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_R0062 LuxR/HSL -35 -10 start BBa_F2620 | 1,54 1,19 20,25 26,44 43,48 63,74 67,70 81,82 81,836 81,830 254,254 572,572 837,861 870,949 870,998 881,924 958,998 965,984 985,998 991,991 1007,1061 1007,1026 1026,1031 1048,1053 1059,1059 1,1061 | feature/BioBrick engineered_region feature/binding non_covalent_binding_site feature/promoter promoter feature/binding non_covalent_binding_site promoter feature/promoter engineered_region feature/BioBrick conserved_region feature/conserved engineered_region feature/BioBrick engineered_region feature/BioBrick feature/cds CDS feature/s_mutation point_mutation feature/s_mutation point_mutation engineered_tag feature/barcode engineered_region feature/BioBrick engineered_region feature/BioBrick stem_loop feature/stem_loop engineered_region feature/BioBrick feature/stem_loop stem_loop feature/polya polyA_site stop_codon feature/stop feature/BioBrick engineered_region non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter start_codon feature/start feature/BioBrick engineered_region |