Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aman Kumar
Date created: 2013-09-25 11:00:00
Date modified: 2015-05-08 01:09:38
pLuxR --> I3A gene
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1194001_sequence (Version 1) |
Description
The I3A gene is actually a polycistronic construct composed of two genes coding for two enzymes - tryptophan transaminase and indole pyruvate decarboxylase. The two DNA constructs are joined by a linker sequence. This entire assembly is placed downstream of an N-acyl homoserine lactone (AHL) inducible promoter pLuxR.The I3A genes function to convert tryptophan to indole-3-acetaldehyde (I3A). Tryptophan transaminase converts tryptophan to indole pyruvate while indole pyruvate decarboxylase converts indole pyruvate to I3A. I3A is a known biofilm inhibitor.
In the future, other teams can use either of our two enzymes as there is a restriction enzyme site incorporated in the linker sequence. The part will be useful in any project involving biosynthesis of indole and its derivatives.
Notes
Lee JH, Kim YG, Kim CJ, Lee JC, Cho MH, Lee, "Indole-3-acetaldehyde from Rhodococcus sp. BFI 332 inhibits E.coli O157:H7 biofilm formation" Appl Microbiol Biotechnol 2012 Nov:96(4):1071-8Source
The gene sequence for tryptophan transaminase is derived from Arabidopsis Thaliana. The gene sequence for indole pyruvate decarboxylase is taken from Azospirillum brasilense. The sequence for pLuxR is taken from Registry.| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0040 TetR 1 -35 TetR 2 -10 BBa_B0034 conserved prefix BBa_C0062 luxR T A Help:Barcodes BBa_B0010 BBa_B0015 stem_loop BBa_B0012 T7 TE polya stop BBa_R0062 LuxR/HSL -35 -10 start BBa_F2620 BBa_K1194001 | 1,54 1,19 20,25 26,44 43,48 63,74 67,70 81,82 81,836 81,830 254,254 572,572 837,861 870,949 870,998 881,924 958,998 965,984 985,998 991,991 1007,1061 1007,1026 1026,1031 1048,1053 1059,1059 1,1061 1070,4113 | engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter non_covalent_binding_site feature/binding feature/promoter promoter feature/BioBrick engineered_region conserved_region feature/conserved engineered_region feature/BioBrick engineered_region feature/BioBrick CDS feature/cds feature/s_mutation point_mutation feature/s_mutation point_mutation feature/barcode engineered_tag engineered_region feature/BioBrick feature/BioBrick engineered_region feature/stem_loop stem_loop feature/BioBrick engineered_region feature/stem_loop stem_loop polyA_site feature/polya stop_codon feature/stop feature/BioBrick engineered_region non_covalent_binding_site feature/binding feature/promoter promoter feature/promoter promoter feature/start start_codon feature/BioBrick engineered_region engineered_region feature/BioBrick |