BBa_K1197000

BBa_K1197000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1197000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2013-09-19 11:00:00
Date modified: 2015-06-08 11:15:20

Anti MazF - Antisense RNA to inhibit MazF activity in B.subtilis



Types
DnaRegion

Roles
RNA

mature_transcript_region

Sequences BBa_K1197000_sequence (Version 1)

Description

Anti MazF is an antisense RNA producing sequence which is complemantary to MazF (K302033). MazF is a non-specific ribonuclease coding element which kills Bacillus subtilis. Anti MazF element produces an mRNA which is reverse complemantary to mRNA produced by MazF. Thus, Anti Mazf mRNA binds to MazF mRNA and inhibit its activity for coding MazF protein.

Notes

This part is designed as a complemantary sequence to the last 4 bases of the RBS sequence, 6 bases to the intermediate region and to the first 40 bases of MazF coding sequence, tottally 50 bases.

Source

It is synthesized as a reverse complementary sequence to MazF (K302033)

igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1197000/1