Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2013-10-01 11:00:00
Date modified: 2015-05-08 01:09:39
CYP6G1 - Insecticide resistance gene
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1197013_sequence (Version 1) |
Description
It is an insecticide resistance gene which is found in Drosophila Melanogaster. We used it in our system to metabolize imidacloprid by producing nontoxic byproducts.It is obtained from Drosophila Melanogaster genome and modified according to work in B.subtilis.
Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)
Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity
of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl.
Acad. Sci. USA 88, 5597e5601.
Notes
Original sequence was modified since it was not proper for prokaryotic expression. Its first 21 nucleotide which coding 7 aa was changed with "Barnes" sequence: MVLTEVL is the native aa sequence, and MALLLAV is the Barnes aa sequence. (Barnes et al. 1991)Reference: Barnes, H.J., Arlotto, M.P., Waterman, M.R., 1991. Expression and enzymatic activity
of recombinant cytochrome P450 17a-hydroxylase in Escherichia coli. Proc. Natl.
Acad. Sci. USA 88, 5597e5601.