Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: safa tapan
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:09:40
Nanofactory Binding Zone (C215)
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1202100_sequence (Version 1) |
Description
This part binds the EpCAM (Epithelial Cell Adhesion Molecule) antigen with great affinity. It is the binding region of our nanofactory part which consists of this region and additional enzyme region.Notes
Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes.Source
This binding region belongs to anti-EpCAM antibody of human body. It is synthesized by GeneScript as the sequence we gave them.| Sequence Annotation | Location | Component / Role(s) |
| J23100 RBS Start Codon His tag linker C215 Stop Codon | 1,35 36,47 48,50 51,68 69,113 114,476 477,482 | promoter feature/promoter feature/rbs ribosome_entry_site feature/start start_codon tag feature/tag engineered_region feature/BioBrick feature/protein CDS feature/stop stop_codon |