Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: safa tapan
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:09:40
MPG-Apoptin: Cancer killing peptide
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1202111_sequence (Version 1) |
Description
this part has two component. Apoptin: forcing cancer cell to apoptosis ,Mpg:signal peptideNotes
Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes.Source
apoptin wss prepare from chicken anemia virus(CAV)| Sequence Annotation | Location | Component / Role(s) |
| RBS Start Codon His Tag J23100 MPG Linker Apoptin Stop Codon | 36,47 48,50 51,68 1,35 69,140 141,185 186,548 549,554 | ribosome_entry_site feature/rbs feature/start start_codon feature/tag tag feature/promoter promoter feature/cds CDS feature/BioBrick engineered_region CDS feature/protein stop_codon feature/stop |