Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: safa tapan
Date created: 2013-09-23 11:00:00
Date modified: 2015-05-08 01:09:40
TAT-RFP
| Types | DnaRegion |
| Roles | engineered_region Translational_Unit |
| Sequences | BBa_K1202113_sequence (Version 1) |
Description
this part designed to tat signal peptide test.Notes
Due to the rare codon analysis, codon optimization is performed for this part. Codons are optimized for E.coli. Additionally, a linker peptide coding region is added for the maximum correctness for our experiments. Lastly, a his tag coding region is added for the purification purposes.Source
tat signal peptide was prepare from Human immunodeficiency Virus-1(HIV-1).RFP sequence was taked parts.igem.org| Sequence Annotation | Location | Component / Role(s) |
| J23100 RBS Start Codon His Tag TAT Linker RFP Stop Codon | 1,35 36,47 48,50 51,68 69,104 105,149 150,830 825,830 | promoter feature/promoter ribosome_entry_site feature/rbs feature/start start_codon feature/tag tag CDS feature/cds engineered_region feature/BioBrick CDS feature/protein feature/stop stop_codon |