BBa_K1204013

BBa_K1204013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1204013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Matthew Baron
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:40

TEF1pr-BS1x-16A



Types
DnaRegion

Roles
Reporter

engineered_region

Sequences BBa_K1204013_sequence (Version 1)

Description

This is a strong constitutive promoter in yeast with a binding site downstream. The binding site is designed to be directly targeted by our iTAL 16A or gRNA 16A. When the binding site is occupied by an iTAL or gRNA/dCas9 complex, the expression of the YFP reporter should be knocked down.

Notes

We had to consider the manner in which iTALs and gRNA/dCas9 complex bind in designing our sequence.

Source

The binding site was synthetically designed using the binding rules for iTALs and gRNA/dCas9 complex and ordered as a gene block from IDT. The TEF1 promoter is a strong constitutive promoter in yeast that we got from another plasmid in the lab.

igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1204013/1