| Types | DnaRegion
|
| Roles | sequence_feature
Other
|
| Sequences | BBa_K1207000_sequence (Version 1)
|
Description
This part is an origin of replication for Gram positive organisms. This sequence has been used in plasmids for electrotransformation of Clostridia with success. It is necessary to have a functional origin of replication for the chassis organism; therefore, if the chassis organism used is a Gram positive, this sequence will be a necessary addition to the iGEM standard plasmid backbones in order to allow for the replication of the plasmid.
Notes
The cloning of this part was relatively straight-forward. No special considerations were required.
Source
This part was cloned from the plasmid pIKM1. Its original source was B. subtilis.
