Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Harland Brandon
Date created: 2013-09-22 11:00:00
Date modified: 2015-05-08 01:09:40
Enhanced Lumazine Synthase (ELS) Expression Construct
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K1210002_sequence (Version 1) |
Description
Lumazine synthase (LS) from Aquifex aeolicus forms icosahedral microcompartment (MC) assemblies of 60 or 180 monomeric units that self assemble and are capable of isolating proteins from their local environment. As shown in previously published work (1), the LS protein has been mutated so that the interior of the MC is negatively charged; the UL 2009 iGEM team has submitted the mutated LS gene to the parts registry (BBa_K249002). A negatively charged interior allows for preferential compartmentalization of positively charged molecules, which can easily be engineered through the addition of a poly-arginine tag to a target protein (1). The size of the cavity of the enhanced Lumazine Synthase microcompartment was enlarged and the negative charge was increased via directed evolution (2). The compartment formed by this polypeptide has a larger cavity and a larger net negative charge, thus allowing a larger loading capacity into the cavity of the compartment.Notes
Part was assembled from intermediate parts.Source
The part was synthesized by Bio Basic Inc. into the pET28a plasmid vector (which allows for expression of ELS with an N-terminal His-tag). The Enhanced Lumazine Synthase (ELS) was moved into the pSB1C3 vector by the Lethbridge 2011 team.| Access | Instance | Definition |
| public public public | BBa_J04500 BBa_K542010 BBa_B0015 | BBa_J04500 BBa_K542010 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J04500 BBa_K542010 BBa_B0015 | 1,220 227,691 700,828 | BBa_J04500 BBa_K542010 BBa_B0015 |