Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anthony Vuong
Date created: 2011-09-25 11:00:00
Date modified: 2015-05-08 01:12:39
Enhanced Lumazine Synthase (ELS)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K542010_sequence (Version 1) |
Description
The part was synthesized by Bio Basic Inc. into the pET28a plasmid vector with an N-terminal His-tag. The Enhanced Lumazine Synthase (ELS) without the His-tag was moved into the pSB1C3 plasmid vector by the Lethbridge 2011 team.The size of the cavity of the Lumazine Synthase microcompartment was enlarged via directed evolution (W??rsd??rfer et al., 2011). The compartment formed by this polypeptide has a larger cavity than the Lumazine Synthase microcompartment submitted by the Lethbridge 2009 team (BBa_K249002). A larger interior allows for the option of localizing larger and/or more proteins into the cavity of the compartment.
Reference:
W??rsd??rfer, B., Woycechowsky, K.J., and Hilvert, D., (2011). Directed Evolution of a Protein Container. Science. 331: 589-592.
Notes
The compartment formed by this polypeptide has a larger cavity than the Lumazine Synthase microcompartment submitted by the Lethbridge 2009 team (BBa_K249002).Source
The Enhanced Lumazine Synthase (ELS) sequence was the result of directed evolution. The part was synthesized by Bio Basic Inc. and moved into pSB1C3 by the Lethbridge 2011 team.W??rsd??rfer, B., Woycechowsky, K.J., and Hilvert, D., (2011). Directed Evolution of a Protein Container. Science. 331: 589-592.