BBa_K1210003

BBa_K1210003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1210003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Harland Brandon

Date created: 2013-10-14 11:00:00

Date modified: 2015-05-08 01:09:40

PK401 tagged dual coding test construct

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1210003_sequence (Version 1)

Description

Reporter construct for measuring the frameshifting efficiency of the PK401 pseudoknot. Includes a His-tagged CFP before the PK401 pseudoknot, and two different polypeptides in the 0 and -1 reading frames with different protein tags.

If translation is halted at the pseudoknot due to the inability to melt the mRNA secondary structure, a 27.7 kDa protein is produced of just CFP-His.
In the 0 frame if read-through of the pseudoknot occurs, a fragment of the IAPV dual coded gene along with a strep-tag II will be translated creating a 33.3 kDa protein of CFP-His-PK401-IAPV-StrepII.
In the -1 frame if read-through of the pseudoknot occurs, a fragment containing the second dual coded IAPV gene along with a GST tag is produced. This contains CFP-His-PK401-IAPV2-GST, a 60.9 kDa protein.

Notes

This construct was designed to verify the applicability of frameshift inducing elements in the programming of protein expression ratios.

Source

This part was synthesized by Genewiz. It contains genomic sequence from IBV virus (PK401 pseudoknot; non-infectious sequence), and from IAPV virusb(IAPV overlapping ORFs; only 7 aa used of the overlapping genes to avoid producing anything harmful).

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1210003/1

Member of these Collections

• iGEM Parts Registry