Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aura Ferreiro
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41
Theophylline Riboswitch (Clone 8.1*)
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1212000_sequence (Version 1) |
Description
Codes for an RNA secondary structure that, in the presence of theophylline, undergoes a conformational change that makes the previously sequestered ribosome binding site available for binding thus allowing for transcription of the downstream sequence. Nominal induction levels for theophylline are 0 to 10 mM. Achieves an 59 - fold activation ratio in 1 mM theophylline as reported by Gallivan and Lynch in 'A flow cytometry based screen for synthetic riboswitches.' Nucleic Acids Res. 2009 January; 37(1): 184???192.Notes
None.Source
Nucleic Acids Res. 2009 January; 37(1): 184???192. Synthetic riboswitch screened from a library of randomized mutants created by cloning theophylline aptamers upstream of the RBS of a B-galactosidase reporter gene where the distance between the aptamer and the RBS was variable.| Sequence Annotation | Location | Component / Role(s) |
| RBS/Shine Dalgarno Theophylline aptamer | 46,49 1,38 | ribosome_entry_site feature/rbs stem_loop feature/stem_loop |