Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Aura Ferreiro
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41
Theophylline Riboswitch (Clone E)
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K1212001_sequence (Version 1) |
Description
Encodes a RNA secondary structure that in the presence of theophylline undergoes a conformational change that makes available the previously sequestered RBS, thus allowing transcription of the downstream sequence. Achieves a 60-fold activation ratio in 2mM theophylline as reported by Topp et al. in: 'Synthetic Riboswitches That Induce Gene Expression in Diverse Bacterial Species.' Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884Notes
None.Source
Appl. Environ. Microbiol. December 2010 vol. 76 no. 23 7881-7884. Synthetic riboswitch generated through rational design and in vivo screening.| Sequence Annotation | Location | Component / Role(s) |
| Theophylline aptamer RBS | 1,38 44,51 | feature/stem_loop stem_loop feature/rbs ribosome_entry_site |