Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Neckelmann
Date created: 2013-09-15 11:00:00
Date modified: 2015-05-08 01:09:41
Golden Gate Compatible pSB3K3
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K1212002_sequence (Version 1) |
Description
The low copy backbone, pSB3K3 has a BsaI site at position 2498. Through site-directed mutagenesis, we altered the base pair 2498 G>A, so that it would be Golden Gate assembly-compatible.Notes
The sequence immediately downstream of the p15a origin in this plasmid is an almost exact match to the AmpR cassette (see BBa_P1002). It seems likely that the orignal definition of the p15a origin was generous and could likely be truncated.Source
The original pSB3K3 backbone was derived from the parts registry.| Sequence Annotation | Location | Component / Role(s) |
| Suffix terminator stem loop E. coli his operon terminator deletion of two bases VR kanR (rev) p15A ORI (rev) VF2 Fixed BsaI Site end of terminator terminator stem loop | 1,21 30,64 22,93 34,35 157,176 239,1054 1386,2210 2611,2630 2498,2503 2687,2690 2700,2719 | engineered_region feature/BioBrick stem_loop feature/stem_loop sequence_feature feature/misc feature/misc sequence_feature primer_binding_site feature/primer_binding CDS feature/cds feature/cds CDS feature/primer_binding primer_binding_site feature/misc sequence_feature feature/misc sequence_feature stem_loop feature/stem_loop |