BBa_K1218025

BBa_K1218025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1218025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Evie Pless

Date created: 2013-09-16 11:00:00

Date modified: 2015-05-08 01:09:42

sacY + RFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1218025_sequence (Version 1)

Description

This composite part contains sacY (BBa_K1218001: a sucrose inducer in Bacillus subtilis), a ribosome binding site (BBa_B0034), and a red fluorescent protein (BBa_E1010). We intend to use it as a chromogenic reporter induced by sucrose.

Notes

None

Source

We constructed the sacY promoter using oligos from Elim, and the RBS and RFP are from the iGEM 2013 Distribution Kit. We used 3A Assembly to construct the composite part.

Components

Access	Instance	Definition
public	BBa_K1218001	BBa_K1218001
public	BBa_B0034	BBa_B0034
public	BBa_E1010	mRFP1

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1218001	1,43	BBa_K1218001
BBa_B0034	52,63	BBa_B0034
BBa_E1010	70,775	mRFP1

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1218025/1

Member of these Collections

• iGEM Parts Registry