Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Orr Schlesinger
Date created: 2013-09-08 11:00:00
Date modified: 2015-05-08 01:09:43
pUC-57-P.A.S.E.2
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1223008_sequence (Version 1) |
Description
P.A.S.E2 cassette cloned into pUC-57 (ampicillin resistance).the cassette is cloned between BamHI and XbaI restriction sites.
Notes
used for amplification and extraction of P.A.S.E2 cassette.Source
genebank, BBa_K354000 (promoter)| Sequence Annotation | Location | Component / Role(s) |
| XbaI site Tyr synthetase genom homology region TAG AraC/Lac promoter (BBa_K354000) Neo/KanR CDS RBS genome homology region BamHI site Rep. origin b-lactamase CDS (ampR) | 425,430 430,490 473,475 512,617 626,1420 497,508 1566,1618 1619,1624 2074,2075 2834,3694 | sequence_feature feature/misc conserved_region feature/conserved sequence_alteration feature/mutation feature/promoter promoter feature/cds CDS ribosome_entry_site feature/rbs feature/conserved conserved_region feature/misc sequence_feature sequence_feature feature/misc CDS feature/cds |