BBa_K1223014

BBa_K1223014 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1223014
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Orr Schlesinger
Date created: 2013-10-02 11:00:00
Date modified: 2015-05-08 01:09:43

tRNA-Pyl (pylT) gene from Methanosarcina barkeri str. Fusaro



Types
DnaRegion

Roles
mature_transcript_region

RNA

Sequences BBa_K1223014_sequence (Version 1)

Description

this is the DNA coding sequence for the tRNA of pyrrolysine from the Archaea Methanosarcina barkeri str. Fusaro.

this tRNA is a part of the machinery that is used to incorporate pyrolysine into proteins in E.coli.

the tRNA synthetase that charges the tRNA with pyrrolysine can be found in biobrick BBa_k1223013.

the tRNA has the anticodon CUA - which means that the sense codon needed to incorporate pyrrolysine is TAG - the amber stop codon.

Notes

this is a part of the pyrrolysine incorporation machinery.

Source

Methanosarcina barkeri str. Fusaro genomic sequence.

Sequence Annotation Location Component / Role(s)
pyrrolysine tRNA CDS
CUA anticodon
1,72
31,33
feature/misc sequence_feature
sequence_feature feature/misc
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