Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Zhang SW
Date created: 2013-09-08 11:00:00
Date modified: 2015-05-08 01:09:43
25aa with T1 terminator and Constitutive promoter veg
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1228006_sequence (Version 1) |
Description
Lf (loctoferrin) is located in many key body fluids , e.g., milk, tears, saliva and other secretions, and in white blood cells ,it has become clear that it is an important part of mammalian defence mechanisms, with a wide range of protective functions.25 amino acid residues is from Lf but has a strong bactericidal, bacteriostatic ability, and the antimicrobial activity than natural LF.We add T1 terminator and Constitutive promoter veg on the 25aa to make it can work regularly.Notes
25aa doesn't contain initiator codon and terminator,so we add a Constitutive promoter veg and T1 terminator .Source
25aa comes from Lf,which was hydrolysed by protease. We synthesis it by Wu Han Bio-broad Co.Ltd| Sequence Annotation | Location | Component / Role(s) |
| BBa_K143012 BBa_K143053 Sigma A-35 Sigma A -10 rbs BBa_K143021 SacB Signal tag BBa_K541501 BBa_K1228004 B0010 BBa_K731722 | 1,97 1,117 63,68 86,91 106,117 106,117 124,219 1,219 228,302 311,389 311,389 | feature/BioBrick engineered_region engineered_region feature/BioBrick promoter feature/promoter feature/promoter promoter feature/rbs ribosome_entry_site engineered_region feature/BioBrick feature/tag tag feature/BioBrick engineered_region feature/BioBrick engineered_region feature/misc sequence_feature engineered_region feature/BioBrick |