Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andr??s Ferri??o Iriarte
Date created: 2013-09-24 11:00:00
Date modified: 2015-05-08 01:09:44
araC-pBad-rbs-LL37-rbs-GFP-pLacI-rbs-MarA Generator
| Types | DnaRegion |
| Roles | Project engineered_region |
| Sequences | BBa_K1230013_sequence (Version 1) |
Description
LL-37 antimicrobial peptide with codon usage for E. coli under the Arabinose promoter with GFP generator.The MarA protein acts as a transcriptional activator in E. coli. It allows the expression of the acrAB and tolC operons, which activate the AcrAB-TolC efflux pump, a mechanism that has been related with resistance to cationic antimicrobial peptides, such as LL-37.
This composite part allows MarA expression in presence of IPTG, by using the LacI promoter and the RBS based on Elowitz repressilator
Notes
In order for this device to work, it has to be transformed into Top 10 cells, the strain for arabinose induction, in which LacI promoter is constitutive.Source
GenScript2013 DNA distribution
| Access | Instance | Definition |
| public public | BBa_K1230010 BBa_K1230004 | BBa_K1230010 BBa_K1230004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1230010 BBa_K1230004 | 1,2244 2253,2871 | BBa_K1230010 BBa_K1230004 |