BBa_K123002

BBa_K123002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K123002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Jason Gardiner

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:09:44

LacIQ ERE TetR

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K123002_sequence (Version 1)

Description

This composite part contains LacIQ (BBa_I14032) and Estrogen Receptor Element (ERE) and TetR (BBa_C0040). The Estrogen Responsive element is to be used in conjunction with ER (Estrogen Receptor). This design involves ER binding to the ERE and disrupting the constituative promoter LacIQ from producing TetR via physically getting in the way of the polymerase.

Notes

There is some data that suggests the ER is toxic to the cell. However in our experiments while we did see a decrease in growth rate we did not find ER to be fatal to the cell.

Source

The Estrogen Responsive Element was taken from the Human Estrogen Responsive Element

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
P(Lac) IQ	1,37	operator, feature/operator
-35	3,8	feature/promoter, promoter
-10	26,31	feature/promoter, promoter
BBa_I14032	1,37	feature/BioBrick, engineered_region
tetR	47,663	CDS, feature/cds
SsrA	664,697	tag, feature/tag
BBa_C0040	44,703	engineered_region, feature/BioBrick
barcode	704,728	engineered_region, feature/BioBrick
ERE	43,57	non_covalent_binding_site, feature/binding

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K123002/1

Member of these Collections

• iGEM 2018 Distribution Plate 6
• iGEM 2018 Distribution Plate 6 Well 4O
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM 2019 Distribution Plate 4
• iGEM 2019 Distribution Plate 4 Well 4O
• iGEM 2019 Part Library
• iGEM 2019 Plasmid pSB1C3
• iGEM Parts Registry