BBa_K125330

BBa_K125330 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K125330
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margaret Ruzicka
Date created: 2008-08-28 11:00:00
Date modified: 2015-05-08 01:09:45

P1 Lytic Replication



Types
DnaRegion

Roles
Temporary

sequence_feature

Sequences BBa_K125330_sequence (Version 1)

Description

The P1 lytic replication region, from Enterobacteria phage P1, contains RepL which initiates replication at origin L (interior to RepL). It is regulated by a lacI promoter. When present in a cell producing LacI, the copy number is <10 copies per cell. If IPTG is added to the media, the promoter is "un-locked", causing the plasmid to be produced at >100+ copies per cell.

Notes

This part is under construction. A terminator (B1006) will be added.

Source

This part was extracted from [[Part:pSB2K3|pSB2K3]] and is described on NCBI at accession number YP_006518.

Sequence Annotation Location Component / Role(s)
RepL
LacOP
279,1127
27,202
CDS feature/cds
sequence_feature feature/misc
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K125330/1