Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Margaret Ruzicka
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:09:45
oriT with lac promoter and lacZ
| Types | DnaRegion |
| Roles | oriT Conjugation |
| Sequences | BBa_K125360_sequence (Version 1) |
Description
This part was constructed to compare the [[Part:BBa_J01003|oriTr]], which is currently available in the registry to [[Part:BBa_K125320|oriT]] which was recently designed to perform the same function. The oriTr was added to [[Part:BBa_J33207|LacZ]] part so that conjugation could be monitored by blue/white selection.Notes
The oriT was synthesized and J33207 was extracted from the registry.Source
The oriT sequence was taken from the self-transmissible plasmid RP4. [[Part:BBa_J33207|LacZ]] is available as a part in the registry.| Access | Instance | Definition |
| public public | BBa_J33207 BBa_K125320 | BBa_J33207 OriT-RP4 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J33207 BBa_K125320 | 1,600 609,707 | BBa_J33207 OriT-RP4 |