nhaS

BBa_K1255000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1255000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Fernanda Puente, Marina Emilio
Date created: 2014-06-12 11:00:00
Date modified: 2015-05-08 01:09:45

nhaS gene that express NhaS, a putative sodium ion binding protein.



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1255000_sequence (Version 1)

Description

"NhaS is proposed to be a sodium binding protein that can enhance the Na+ -resistance of antiporter- deficient strains by increasing the availability of Na+ to the integral membrane antiporters on the cytoplasmic side of the membrane and by sequestering Na+ while rate-limiting efflux mechanisms catalyze extrusion of the cation." (Krulwich et al. 1994)

"The NhaS protein enhances resistance of bacteria into which the nhaS gene has been introduced, to Na+ by sequestering Na+ and reducing its cytotoxicity." (Krulwich et al. 1994)

NhaS is protein that binds to sodium ions and enhance the bacteria's resistance up to 15% to high saline conditions.

Notes

The original genomic sequence was optimized for expression in E. coli.

IVEY Mark, KRULWICH Terry. (1994) Sodium ion binding proteins. From http://www.google.com.mx/patents/US5346815.

Source

NhaS comes from Bacillus Firmus.

Sequence Annotation Location Component / Role(s)
nhaS
1,207
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1255000/1