Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: İBRAHİM YASİR ORHAN
Date created: 2014-06-22 11:00:00
Date modified: 2015-05-08 01:09:46
HORSERADISH PEROXIDASE TYPE C COMPOSITE PART
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1291071_sequence (Version 1) |
Description
The enzyme horseradish peroxidase (HRP), found in the roots of plant horseradish, is used extensively in biochemistry applications primarily for its ability to amplify a weak signal and increase detectability of a target molecule. It is a metalloenzyme with many isoforms and the most studied type is C. This enzyme is primerly responsible for the degradation of industrial dyes such as Methylene Blue.The composite part starts off with the J23119 constitutive promoter, continues with the RBS which is followed by the Horseradish peroxidase coding region. The sequence ends with the terminators which come after the Histag region. There is the BamH1 enzyme restriction cite on the sequence before the RBS.
Notes
All EcoRI, PstI, SpeI, XbaI restriction sites were removed manually and BamH1 restriction cites were placed at the start and at the end of the composite part.Source
Horseradish peroxidase Type C region was taken from the horseradish and the other compositions were synthetically merged with this sequence.| Access | Instance | Definition |
| public public public | BBa_J23119 BBa_B0034 BBa_K1291571 | BBa_J23119 BBa_B0034 BBa_K1291571 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23119 BBa_B0034 BBa_K1291571 | 1,35 44,55 62,1120 | BBa_J23119 BBa_B0034 BBa_K1291571 |