Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kyoungwoo Jang
Date created: 2014-06-04 11:00:00
Date modified: 2015-05-08 01:09:46
Klebsiella oxytoca Urease
| Types | DnaRegion |
| Roles | Plasmid plasmid |
| Sequences | BBa_K1297000_sequence (Version 1) |
Description
This part is complex of pSB1C3 vector and urease gene from Klebsiella oxytoca KCTC 1686.Our main purpose is prevention of desertification by biocementation, and we used this enzyme for biocementation. A function of urease is catalyzing the hydrolysis of urea to release ammonia and carbon dioxide. The released carbon dioxide dissolves in water, and is precipitated with Ca2+, forming CaCO3 (biocement).
In our study, we cloned this enzyme with pBAD vector by ligation independent cloning (LIC) for enzyme overexpression, and observed the catalytic activity of it in Escherichia coli by pH indicator assay. To verify microbial biocementation, we performed a biocementation test with synthetic E. coli and detected the whole-cell activity by measuring the radius of precipitated biocement.
Notes
A size of the urease is very big (about 5 kb) because this gene has many subunits.Source
genomic DNA of Klebsiella oxytoca KCTC 1686| Sequence Annotation | Location | Component / Role(s) |
| UreD urease_gamma urease_beta urease_alpha UreE UreF urease_accessory | 1,825 834,1137 1147,1459 1460,3163 3173,3646 3637,4321 4330,4947 | CDS feature/protein CDS feature/protein CDS feature/protein feature/protein CDS feature/protein CDS CDS feature/protein feature/protein CDS |