Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Kevin McLeod
Date created: 2008-07-21 11:00:00
Date modified: 2015-05-08 01:09:47
ColE2 SOS Promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K131001_sequence (Version 1) |
Description
This promoter normally represses the ColE2 activity gene, ceaB, in the ColE2 operon.To activate this promoter would require a RecA positive strain, as the protein LexA represses the promoter. To induce this promoter, an intercalating agent, mitomycin C, was utilized to induce the SOS response, and thus the expression of the genes under control of this promoter.
Notes
Lab strains of E. coli, such as Top10 from Invitrogen, are deficient in RecA so we required a RecA positive strain to induce this promoter. We used BL21 from Novagen.Source
This part comes from the ColE2 operon, which came from a plasmid.| Sequence Annotation | Location | Component / Role(s) |
| -35 Signal -15 Signal Region of Dyad Symmetry SOS Box | 218,224 241,246 136,161 252,267 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature |