BBa_K131010

BBa_K131010 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K131010

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kevin McLeod

Date created: 2008-07-27 11:00:00

Date modified: 2015-05-08 01:09:47

AHL Inducible Colicin E2 with GFP

Details

• Types: DnaRegion
• Roles: Generator; engineered_region
• Sequences: BBa_K131010_sequence (Version 1)

Description

This part is inducible with AHL to fluoresce green and activate the colicin genes (K131009).

Notes

GFP (I13500) had to be placed before the colicin E2 operon because of the intrinsic terminator present in the operon. Otherwise little to no GFP would be produced.

Source

F2620 and I13500 were preexisting parts from the registry. See K131000 for information on the colicin genes.

Components

Access	Instance	Definition
public	BBa_F2620	BBa_F2620
public	BBa_I13500	BBa_I13500
public	BBa_K131009	ColE2 - SO

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_F2620	1,1061	BBa_F2620
BBa_I13500	1070,1807	BBa_I13500
BBa_K131009	1816,4144	ColE2 - SO

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K131010/1

Member of these Collections

• iGEM Parts Registry