Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Thane Kubik
Date created: 2008-09-27 11:00:00
Date modified: 2015-05-08 01:09:47
LuxPQ from Vibrio harveyi
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K131015_sequence (Version 1) |
Description
LuxPQ from Vibrio harveyiLuxP is a ABC-type sugar transport system periplasmic protein (Protein ID = YP_001447483.1).
LuxQ is a histidine kinase (Protein ID = YP_001447484.1).
Notes
LuxP and LuxQ are found in the operon, thus we decided to keep them together. We kept natural RBS in front of LuxP, as well as the intrinsic terminator. Silent mutations were performed on a EcoRI site located within LuxP, EcorI site within LuxQ, and XbaI site within LuxQ.Source
GenBank Accession: NC_009784Vibrio harveyi ATCC BAA-1116 chromosome II, REGION: 579654..580751.
Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM. Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing. Cell. 2006 Sep 22;126(6):1095-108.
Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM.
| Sequence Annotation | Location | Component / Role(s) |
| Start of LuxP Stop for LuxP Start of LuxQ Stop for LuxQ LuxP LuxQ Silent mutation to remove EcoRI site Silent mutation to remove EcoRI site Silent mutation to remove XbaI site | 30,32 1125,1127 1127,1129 3704,3706 30,1127 1127,3706 599,599 1465,1465 1618,1618 | feature/start start_codon stop_codon feature/stop feature/start start_codon feature/stop stop_codon feature/cds CDS feature/cds CDS point_mutation feature/s_mutation point_mutation feature/s_mutation feature/s_mutation point_mutation |