BBa_K1311003

BBa_K1311003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1311003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinye Wang
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:47

promoterK081005+TALE-N_terminal-LacZ-C_terminal+terminatorB0015



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1311003_sequence (Version 1)

Description

Our team use this part as a final vector for TALE(TranscriptionActivator???Like Effectors, TALE) assembly via Golden Gate Assembly. When we insert the TALE repeats into this vector, the reporter gene LacZ will be knocked out by the restriction enzyme Esp3I. Then we can use blue-white screening to select the right white colonies by add 40μL of 20mg/mL X-gal +40μL of 0.1M IPTG into the plate.

Notes

The N-terminal of TALE contains the restriction sites of PstI. Therefore, users should note if they want to use PstI to digest this plasmid.

Source

natural TALE sequence

Sequence Annotation Location Component / Role(s)
BBa_J23100
BBa_K081005
RBS-1\Strong
RBS
N-terminal
Reporter_LacZ
lacZ_promoter
C-terminal
1,35
1,58
44,58
51,55
65,893
952,1112
1273,1302
1380,2165
feature/BioBrick engineered_region
feature/BioBrick engineered_region
feature/rbs ribosome_entry_site
ribosome_entry_site feature/rbs
sequence_feature feature/misc
feature/misc sequence_feature
feature/promoter promoter
feature/misc sequence_feature
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1311003/1