Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinye Wang
Date created: 2014-10-08 11:00:00
Date modified: 2015-05-08 01:09:47
TetR inducible promoter+TALE N-terminal +LacZ+TALE C-terminal
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1311004_sequence (Version 1) |
Description
We can use this part to control the expression of TALE (TranscriptionActivator???Like Effectors, TALE) if we add tetracycline or its analog, aTc into the bacteria culture. There is no difference between part BBa_K1311003 and part BBa_K1311004 except the promoter. The promoter of this part is pTet, which can be suppressed by TetR.Notes
There is no RBS in the downstream of pTet. The users must insert RBS between pTet and TALE N-terminal before they want to use this plasmid to express TALE.Source
Natural TALE Sequence| Sequence Annotation | Location | Component / Role(s) |
| J23101 TetR(C0040) RBS-strong (B0030) TetR1 TetR2 TALE N-terminal Reporter LacZ lac promoter TALE C-terminal Promoter B0015 B0015 | 1,35 70,689 44,58 897,915 922,941 957,1785 1844,2004 2165,2194 2272,3057 938,944 760,888 3068,3196 | engineered_region feature/BioBrick feature/dna sequence_feature feature/rbs ribosome_entry_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter feature/misc sequence_feature feature/promoter promoter feature/BioBrick engineered_region feature/BioBrick engineered_region |