Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Carlos Meza
Date created: 2014-10-11 11:00:00
Date modified: 2016-01-28 01:43:08
Neomycin Resistance
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1313004_sequence (Version 1) |
Description
Neomycin resistance gene. This gene can be used for selection of cells with an efficient transformation. This gene codes neomycin phosphotransferase which breaks neomycin, kanamycin, and gentamicin molecules. If you use these gene as selectivity gene in bacteria, and your laboratory lacks neomycin, you can replace it for kanamycin by adding 35 mg/ml per liter of LB media. The best concentration of neomycin to use is 80 mg/ml per liter of LB media.This gene can be also used as a selective gene for mammalian cells using gentamicin.