BBa_K1314000

BBa_K1314000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1314000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Seak Chi U, Chan Wai Lun, CHIU, Yee Ting

Date created: 2014-10-05 11:00:00

Date modified: 2015-05-08 01:09:47

Ammonium repressible nifH promoter(from Azotobacter vinelandii)

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1314000_sequence (Version 1)

Description

This part arose from the natural version of the A. vinelandii nifH promoter with illegal restriction site removed. It is part of the transcriptional regulatory system of the nitrogenase of A. vinelandii. The expression level of the gene under this promoter was found to be highest when nitrogen is depleted in A. vinelandii.(Trinity L. et al. , 2011) With a sufficiently low nitrogen level, the expression level from nifH promoter protein expression system is expected to be higher than that in tranditional E. coli T7 protein expression system. (2)

Notes

none

Source

A. vinelandii(strain DJ)

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
nifH promoter	1,220	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1314000/1

Member of these Collections

• iGEM Parts Registry