BBa_K1315001

BBa_K1315001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1315001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dimitrios Michailidis
Date created: 2014-09-19 11:00:00
Date modified: 2015-05-08 01:09:48

The pqsA promoter from Pseudomonas



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_K1315001_sequence (Version 1)

    Description

    The Pseudomonas quinolone signal (PQS) is imperative in modulating mechanisms that enable Pseudomonas aeruginosa to survive at times of stress. P. aeruginosa is the main pathogen responsible for severe deterioration in the Cystic Fibrosis lungs. The production of this signal relies on the pqsABCDE operon. The transcriptional factor PqsR (BBa_K1315000) positively regulates this operon.

    http://www.ncbi.nlm.nih.gov/pubmed/25225275
    http://www.ncbi.nlm.nih.gov/pubmed/16735731
    http://www.ncbi.nlm.nih.gov/pubmed/21539583
    http://www.ncbi.nlm.nih.gov/pubmed/?term=pqsr-dependent+and+pqsr-independent+regulation+of+motility+and+biofilm+formation+by+pqs
    http://www.ncbi.nlm.nih.gov/pubmed/12393198

    Notes

    The sequence did not have any restriction sites to remove so it was immediately PCRed out of the genome and had the registry prefix and suffix added to it. It was then ligated into the pSB1C3 vector.

    Source

    The source of this part is the genome of Pseudomonas aeruginosa PAO1 provided by the Robert Ryan lab in the Molecular Microbiology department of the University of Dundee, UK.

    Sequence Annotation Location Component / Role(s)
    pqsABCDE
    		1,92
    		promoter feature/promoter

    igem#experience
    None
     
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1315001/1
     

    Attachments

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