BBa_K1315009

BBa_K1315009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1315009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Dimitrios Michailidis
Date created: 2014-09-25 11:00:00
Date modified: 2015-05-08 01:09:50

Pseudomonas AutoInducer 1 (PAI-1) Biosensor



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1315009_sequence (Version 1)

Description

This is a composite part that consists of 4 biobricks. In the front it has ptet(Bba_R0040) which is constitutively on. This produces LasR all the time with lasR(Bba_C0179) next in the sequence. Only in the presence of the Pseudomonas Autoinducer 1 (PA1), LasR forms an complex with PA1 and it can then activate the plasB(Bba_R0079) promoter that leads to the production of GFP(Bba_E0040).

Notes

All the inserts were digested with XbaI and PstI, and all the vectors were each time cut with SpeI and PstI.

Source

All the parts have come from existing iGEM distribution kits.

igem#experience Works  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_K1315009/1