Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-11 11:00:00
Date modified: 2015-05-08 01:09:50
Rhamnose promoter coupled to N-genes
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Truncate Labels
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1316006_sequence (Version 1) |
Description
nfsA, nfsB and nemA (refered here as N-genes) are the genes for NfsA, NfsB and NEM reductases, which presumably play a role in 2,4-DNT and 2,4,6-TNT metabolism. These genes are regulated by the inducible Rhamnose promoterNotes
The Rhamnose promoter was used to inducibly regulate the expression of the N-genesA strong RBS was placed in front of each gene
An "all frame STOP" region was placed after each gene
nfsA and nfsB have a 6xHis tag at the N-terminus; nemA has a 6xHis tag at the C-terminus. This way, the proteins can be purified to check if they are successfully expressed
Source
"De novo" synthesis| Sequence Annotation | Location | Component / Role(s) |
| Rhamnose promoter RBS B0034 nfsA 6xHis tag stop RBS B0034 nfsB stop 6xHis tag RBS B0034 nemA 6xHis tag stop His terminator start start start | 1,122 129,140 149,887 152,170 888,891 897,909 916,1585 1585,1588 919,937 1600,1612 1619,2732 2714,2732 2733,2735 2737,2795 149,152 916,918 1619,1621 | promoter feature/promoter ribosome_entry_site feature/rbs feature/cds CDS feature/tag tag feature/stop stop_codon feature/rbs ribosome_entry_site feature/cds CDS stop_codon feature/stop tag feature/tag ribosome_entry_site feature/rbs feature/cds CDS feature/tag tag feature/stop stop_codon feature/dna sequence_feature start_codon feature/start start_codon feature/start start_codon feature/start |