Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:09:50
pRhamnose regulating csgB + J23110 constitutive Anderson promoter regulating csgA with a His tag
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1316014_sequence (Version 1) |
Description
This part is meant to constitutively express the curli gene csgB which codes for the production of the curli protein that is anchored to the cell membrane (CsgB).The csgA gene codes for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure.
As many more CsgA than CsgB subunits are required for curli formation, this part intends to have an already high amount of CsgA present in the cell (constitutively expressed) and, once the cell is induced (with Rhamnose in this case), only CsgB protein is required to be expressed for curli formation, thus leading to a faster curli generation process.
The His tag present in the CsgA protein has two functions: on one hand it allows easy protein purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive.
Notes
Many more CsgA protein subunits are required compared to CsgB in curli formation.A His tag was added into the CsgA protein for the mentioned purposes (The His tag present in the CsgA protein has two functions: on one hand it allows easy protein purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive.)
Source
csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part.| Access | Instance | Definition |
| public public | BBa_K1316018 BBa_K1316020 | BBa_K1316018 BBa_K1316020 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1316018 BBa_K1316020 | 1,535 544,1058 | BBa_K1316018 BBa_K1316020 |