Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:09:50
2nd part of Constitutive Anderson promoter + csgA:Histag curli gene
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1316020_sequence (Version 1) |
Description
Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgA is the major curli subunit.A His tag was added at the C-terminus of csgA's coding sequence. This His has two functions: on one hand it allows easy purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive.
Notes
The part was PCRed in a way that it contains the ending of a constitutive promoter right before the open reading frame (ORF) of csgA gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.A His tag was added at the C-terminus of csgA's ORF.