Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:09:50
pRhamnose regulating csgB and csgA curli proteins
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K1316015_sequence (Version 1) |
Description
This part is meant to express the csgB and csgA curli genes, which respectively code for the production of the curli protein that is anchored to the cell membrane (CsgB); and for the major curli protein (CsgA), which forms long fibrils with other CsgA monomers in a self-assembly process. These CsgA long fibrils bind to the anchored CsgB protein forming the amyloid-like curli structure.The expression of these two genes will, then, occur at the same time and under the same conditions (in the presence of Rhamnose in this case)
This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formation
Notes
This construct was designed as a negative control for BBa_K1316013 and BBa_K1316014, to see if expressing CsgA constitutively has a positive impact on the speed of curli formationSource
csgA and csgB were PCRed out of the K540000 Lyon 2011 iGEM part.| Sequence Annotation | Location | Component / Role(s) |
| Rhamnose promoter rbs csgB start stop csgA start stop | 1,122 148,159 161,616 161,163 614,616 657,1112 657,659 1110,1112 | feature/promoter promoter ribosome_entry_site feature/rbs CDS feature/cds start_codon feature/start stop_codon feature/stop feature/cds CDS feature/start start_codon feature/stop stop_codon |