BBa_K1316019

BBa_K1316019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1316019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Joan Cortada Garcia
Date created: 2014-10-15 11:00:00
Date modified: 2015-05-08 01:09:50

2nd part of Constitutive Anderson promoter + csgA curli gene



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1316019_sequence (Version 1)

Description

Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgA is the major curli subunit.

Notes

The part was PCRed in a way that it contains the ending of a constitutive promoter right before the open reading frame (ORF) of csgA gene. This aims at constructing via Golden Gate Assembly the final BioBricks BBa_K1316013 and BBa_K1316014 where csgB and csgA genes are present and a constitutive promoter was placed between them.

Source

The CDS of csgA contained in this part was PCRed from the iGEM BioBrick BBa_K540000 Lyon team 2011.

Sequence Annotation Location Component / Role(s)
Ending constit Promoter
rbs
csgA
stop
start
1,13
14,25
27,479
477,479
27,29
feature/promoter promoter
feature/rbs ribosome_entry_site
CDS feature/cds
feature/stop stop_codon
start_codon feature/start
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1316019/1