BBa_K1317001

BBa_K1317001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1317001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Mounir Benkoulouche
Date created: 2014-10-06 11:00:00
Date modified: 2015-05-08 01:09:50

CDS for resilin-like polypeptide (RLP)



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1317001_sequence (Version 1)

Description

This part is the coding sequence for the resilin like polypeptide. This sequence was assembled from a consensus of the proresilin exon 1 from Drosophila melanogaster.
This protein, in insects, allows resistance and elasticity used for jumping, flapping...
The synthetic gene encodes a synthetic protein "resilin-like". The repeated aminoacids allow to retrieve these properties of resistance, resilience and elasticity, but with a minimal pattern of the original protein, which is more suited for downstream applications.
It can be used to produce wire presenting these properties after wet-spinning.

Notes

The gene was synthesized with oligos specially designed for PCR fusion and Gibson Assembly.

Source

Genomic sequence from Drosophila melanogaster

Sequence Annotation Location Component / Role(s)
Start codon
Stop Codon
RLP
aromatic (dosage)
ChewBack
NheI
6,8
318,320
6,320
9,11
306,317
299,305
start_codon feature/start
stop_codon feature/stop
CDS feature/cds
feature/tag tag
sequence_feature feature/misc
engineered_region feature/BioBrick
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BBa_K1317001/1