REACh1

BBa_K1319001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1319001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Osthege, Florian Gohr

Date created: 2014-09-02 11:00:00

Date modified: 2015-05-08 01:09:50

RFC[25]-compatible dark quencher based on K1319000 (E0030)

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1319001_sequence (Version 1)

Description

This part is a RFC[25] dark quencher that is based upon K1319000 (the RFC[25] version of E0030/EYFP).

Two mutations were introduced that eliminated fluorescence:

• L90I
• Y145W

=References=

• Ganesan S, Ameer-Beg SM, Ng TT, Vojnovic B, Wouters FS. A dark yellow

fluorescent protein (YFP)-based Resonance Energy-Accepting Chromoprotein (REACh)
for F??rster resonance energy transfer with GFP. Proc Natl Acad Sci U S A. 2006
Mar 14;103(11):4089-94. Epub 2006 Mar 6. PubMed PMID: 16537489; PubMed Central
PMCID: PMC1449651. PubMed Central

Notes

The Y145W mutation was introduced to eliminate fluorescence of the protein.

Source

The part was created by site-directed mutagenesis on K1319000 that we previously created from E0030

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
REACh1	1,711	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1319001/1

Member of these Collections

• iGEM Parts Registry