BBa_K1319009

BBa_K1319009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1319009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege
Date created: 2014-10-01 11:00:00
Date modified: 2015-05-08 01:09:50

mRFP-gal3-his fusion protein CDS



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1319009_sequence (Version 1)

Description

This is a fusion protein created from E1010, K1319003 and K1319007.

Notes

The stop codon and barcode sequence was removed from E1010.
We assembled the DNA by Gibson assembly, but designed the sequence to contain RFC[25] scars (ACCGGC) between the domains.

Source

it's a combination of other part sequences.

Sequence Annotation Location Component / Role(s)
start
stop
RFC25 scar
RFC25 scar
mRFP
galectin-3
6xHis
1,3
1453,1458
765,770
1429,1434
1,764
771,1428
1435,1452
feature/start start_codon
stop_codon feature/stop
feature/scar
feature/scar
feature/cds CDS
CDS feature/cds
tag feature/tag
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1319009/1