BBa_K1321301

BBa_K1321301 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1321301

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Michael Florea

Date created: 2014-10-07 11:00:00

Date modified: 2015-05-08 01:09:52

pSEVA321-Bb

Details

• Types: DnaRegion
• Roles: plasmid_vector; Plasmid_Backbone
• Sequences: BBa_K1321301_sequence (Version 1)

Description

This is pSEVA321 converted into a biobrick compatible format by substituting the original multiple cloning site for biobrick prefix and suffix. pSEVA321 is a broad host range plasmid, capable of replication in E.coli and several other organisms. It is capable of replication in the cellulose producing bacterium Gluconacetobacter xylinum. We have used it as our standard backbone for G.xylinum genetic engineering.

Notes

None

Source

The original pSEVA331 backbone comes from the SEVA (Standard European Vector Architecture) collection.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Biobrick suffix	1,21	sequence_feature, feature/misc
Biobrick prefix	3572,3593	sequence_feature, feature/misc
Chloramphenicol acetyltransferase	271,930	feature/protein, CDS
OriT	954,1189	feature/misc, sequence_feature
Replication protein TrfA	1248,2396	feature/misc, sequence_feature

Other Properties

• igem#experience: Works
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1321301/1

Member of these Collections

• iGEM Parts Registry
• igem_plasmid_vectors